
Making Real Tissue Reality
100% Cellular Tissue
TissueByNet is enabling researchers to successfully create tissues composed entirely of cells.
High-functioning, robust, and pure tissue unaffected by restrictive polymers or hydrogels are now easily created using TissueByNet Net-Engineered Tissue Technology.
NETT: Net-Engineered Tissue Technology
A Paradigm Shift in Process and Outcome
Unlike traditional 3D bioprinting extrusion techniques, TissueByNet embraces a process more likened to “bioassembly.”
By utilizing cellular spheroid constructs as intermediate building blocks between singular cells and viable tissue, TissueByNet's Net Mold technology facilitates ECM production solely through cultured cells.
These spheroids consisting purely of cultured cells mature within the net mold, simulating a more natural growth process and creating unadulterated, robust and high-functioning tissue *.
* NETT has been proven to create tissue with increased cell density, higher cell viability, as well as enhanced function and expression compared to bioprinted gel mold tissue s.
1


Results: Pure Cellular Tissue,
40mm Tube, Suturble Tissue
Net Mold construction
Step 1: (Spheroid Making)
Pour Cell Suspension into Dimple Plate and culture overnight.
Thousands of Spheroids made with Dimple Plate.
Shape of Spheroid is not a problem as long as they are large enough to be trapped by Net Mold.


Step 2: (Feed into Net Mold)
Feed Spheroids into Net Mold.
This is done manually by hand.
Culture in medium for 1-3 weeks.

Step 3: (Remove Nets)
Medium has access to cells through net spacing, enabling sufficient culture conditions for cells. Net Mold cavity holds spheroids in contact each other and secreted ECM helps cell fusion.
After culture completion, Net can be slide out easily from Cell Block.
