
Making Real Tissue Reality
3D Cellular Block
by
Net Mold Technology
Fabricate 3D Cell Block in simple procedure without bioprinter.
Net Mold Process
Culture Cells in assembled "Net" to help cell to fuse each other.
Some Cell types have nature to adhere to each other. Net Mold is a device to help cells to adhere each others and become a Cell Block.
Net Mold an assembled "Net" which has cavity to hold cell spheroids within. As Cell spheroids are trapped by net, media will have access to cells through net opening, while spheroids are fuse together and become one large Cell Block.
Unlike conventional 3D bioprinters that use bio-inks (gels containing cells, etc.) which block cell-to cell direct fusion, cells adhere directly each others in Net Mold.
3D cell blocks by the Net Mold method are strong enough to suture and also produce many kind of cytokines such as TGF-B1.
Net Mold is effective for human, animals and plants as long as it is an adhesive cell.


Video
Overview of the Net Mold Method
Chicken Cell-Cultured Meat
Not edible yet
Process

Step 1: Prepare spheroids
Thousands of spheroids can be produced at once by Dimple Plate
Spheroids are "intermediate materials", so any shape can be used as long as they can be trapped by the net.
Step 2: Feed Spheroid into Net Mold
Pipette the spheroid into the net mold cavity.
After feeding, incubate in the medium for about 3 weeks.
Step 3: Remove the Net
During the culture, cell secrete collagen which is essential for fusion.
Spheroids fuse together in Net Mold cavity and they become one Cell Block.
The cell block is strong enough to be handled with tweezers and sutured.



