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Making Real Tissue Reality

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Making Real Tissue Reality

3D Cellular Block
by 
Net Mold Technology

Fabricate 3D Cell Block in simple procedure without bioprinter.

Net Mold Process
Culture Cells in assembled "Net" to help cell to fuse each other.

Some Cell types have nature to adhere to each other.  Net Mold is a device to help cells to adhere each others and become a Cell Block.

Net Mold an assembled "Net" which has cavity to hold cell spheroids within.  As Cell spheroids are trapped by net, media will have access to cells through net opening, while spheroids are fuse together and become one large Cell Block.

Unlike conventional  3D bioprinters that use bio-inks (gels containing cells, etc.) which block cell-to cell direct fusion, cells adhere directly each others in Net Mold.

 

3D cell blocks by the Net Mold method are strong enough to suture and also produce many kind of cytokines such as TGF-B1.

Net Mold is effective for human, animals and plants as long as it is an adhesive cell.

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Video

Overview of the Net Mold Method

Chicken Cell-Cultured Meat

Not edible yet

 

Process

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Step 1: Prepare spheroids

Thousands of spheroids can be produced at once by Dimple Plate

Spheroids are "intermediate materials", so any shape can be used as long as they can be trapped by the net.

Step 2: Feed Spheroid into Net Mold

 

Pipette the spheroid into the net mold cavity.

 

After feeding, incubate in the medium for about 3 weeks.

Step 3: Remove the Net

During the culture, cell secrete collagen which is essential for fusion.

Spheroids fuse together in Net Mold cavity and they become one Cell Block.

The cell block is strong enough to be handled with tweezers and sutured.

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